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ORIGINAL ARTICLE
Year : 2021  |  Volume : 16  |  Issue : 1  |  Page : 33-35

The screening and identification of atypical red cell antibodies by simultaneous LISS/coombs and NaCl/enzyme gel methods prior to blood transfusion


Department of Pathology, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences (Deemed to be University), Wardha, Maharashtra, India

Correspondence Address:
Dr. Purvee Agrawal
F-7 Meghe Heights, Jawaharlal Nehru Medical College Campus, Sawangi, Meghe, Wardha, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1319-4534.322597

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Background: In this retrospective study, we measured the frequency of unexpected antibodies in the blood by Nacl/enzyme gel method or LISS/Coombs method and compared the result and frequency of identification in patients with a history of gestation or blood transfusion. Aims and Objectives: To detect unexpected/atypical red cell antibodies by simultaneous LISS/Coombs and NacL/enzyme gel methods prior to blood transfusion. Materials and Methods: Atypical antibody screening has done in all patients with the history of recurrent blood transfusion or history of gestation by commercially available red cell panel (ID–Dia panel, Diamed–ID Micro Typing System). Results: A total of 30 patients were screened for the presence of atypical antibody. Out of 30 patients, 1 (3.33%) patient showed the presence of alloantibody in the serum. The alloantibody identified was of anti-Lewis system. Conclusion: Screening for the presence of alloantibodies in patients is important to provide compatible blood and avoid adverse transfusion reactions.


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