• Users Online: 414
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
Year : 2020  |  Volume : 15  |  Issue : 1  |  Page : 114-117

Influence of combination of docosahexaenoic acid supplement and a polyherbal formulation (Liv. 52) on carbon tetrachloride-induced hepatic injury: A preclinical study

1 Department of Pharmacology, Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal, Karnataka, India
2 Department of Pharmacology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras al Khaimah, UAE
3 Melaka Manipal Medical College, Jalan Padang Jambu, Bukit Baru, 75150 Melaka, Malaysia
4 Department of Pharmacology, Raipur Institute of Medical Sciences, Raipur, Chhattisgarh, India

Correspondence Address:
Dr. Shakta Mani Satyam
Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal - 576 104, Karnataka
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jdmimsu.jdmimsu_219_19

Rights and Permissions

Background: Docosahexaenoic acid (DHA) has diverse functions in normal metabolism and health and are widely used as a nutritional supplement. Liv. 52 is a polyherbal formulation used in hepatic dysfunction. The present study was undertaken to investigate the influence of oral supplementation of Liv. 52 and DHA alone and their combination against carbon tetrachloride (CCl4) induced hepatic injury in Wistar rats. Materials and Methods: Hepatotoxicity was induced by administering 1:1 mixture of CCl4and olive oil; 1 ml/kg/72 h i.p. A total of 54 adult female Wistar (150–200 g) rats were divided into nine groups of six rats each as follows-Group 1-Normal healthy control (1 ml/kg/day of 2% gum acacia), Group 2: Negative control (CCl4 +1 ml/kg/day of 2% gum acacia), Group 3: Positive control (CCl4+ Silymarin 50 mg/kg/day), Group 4-9: CCl4+ Liv. 52–225 mg/kg/day, 450 mg/kg/day, DHA-300 mg/kg/day, 600 mg/kg/day alone and their combination. The treatment duration was 7 days. Hepatoprotective potential was studied by the estimation of serum alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) in experimental rats. Results: Serum ALT, AST, and ALP were significantly increased (P < 0.05) in hepatotoxic control rats compared to normal healthy control rats. There was statistically significant change (P < 0.05) in serum levels of ALT, AST, and ALP among Silymarin, Liv. 52 and DHA treated rats in comparison to hepatotoxic control rats.Conclusions: The present study revealed that Liv. 52 and DHA alone and in combination ameliorates the hepatic injury induced by CCl4in Wistar rats.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded12    
    Comments [Add]    

Recommend this journal