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ORIGINAL ARTICLE
Year : 2018  |  Volume : 13  |  Issue : 3  |  Page : 122-129

Immunohistochemical evaluation of expression pattern of p53, p63, and p73 in epithelial dysplasia


1 Department of Oral Pathology and Microbiology, SDK Dental College and Hospital, Wanadongri, Nagpur, Maharashtra, India
2 Department of Oral Pathology and Microbiology, SPDC, Sawangi, Maharashtra, India
3 Department of Oral Pathology and Microbiology, CDCRH, Rajnandgao, Chhattisgarh, India
4 Department of FMT, JNMC, Wardha, Maharashtra, India

Correspondence Address:
Dr. Deepali Prakash Mohite
Plot No. 139, Ganesh Nagar, Sharda Chowk., Nagpur - 440 009, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jdmimsu.jdmimsu_64_18

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Objective: The objective of this study was to evaluate the expression pattern of p53, p63, and p73 in different grades of epithelial dysplasia through immunohistochemical (IHC). Materials and Methods: 30 cases of epithelial dysplasia and 10 samples of normal buccal mucosa were included in the study. The cases with epithelial dysplasia were classified as mild, moderate and severe. Immunohistochemical staining was carried out using monoclonal mouse anti-human antibody against p53, p63, and rabbit polyclonal antibody against p73. Labelling index was calculated. The numbers of positively stained nuclei were expressed as a percentage of the total number counted for individual layer and in complete epithelium. The number of cells positive for p63 protein immunoexpression was higher than that for p53. Significant difference in positivity was observed with respect to p53 and p63 amongst all the grades of dysplasia. In severe dysplasia, significantly higher expression of p63 was observed. Expression of p53 was significantly higher in severe dysplasia than mild dysplasia and normal tissue. p73 was observed to be non significant amongst all grades of dysplasia and in normal tissue. Significant positive correlation was noted between p63 and p53 in severe dysplasia. Study Design: Thirty cases of epithelial dysplasia and 10 samples of normal buccal mucosa were included in the study. The cases with epithelial dysplasia were classified as mild, moderate, and severe. IHC staining was carried out using monoclonal mouse anti-human antibody against p53, p63, and rabbit polyclonal antibody against p73. Labeling index was calculated. The numbers of positively stained nuclei were expressed as a percentage of the total number counted for the individual layer and in the complete epithelium. The number of cells positive for p63 protein immunoexpression was higher than that for p53. Significant difference in positivity was observed with respect to p53 and p63 among all the grades of dysplasia. In severe dysplasia, significantly higher expression of p63 was observed. Expression of p53 was significantly higher in severe dysplasia than mild dysplasia and normal tissue. p73 was observed to be nonsignificant among all grades of dysplasia and in normal tissue. Significant positive correlation was noted between p63 and p53 in severe dysplasia. Results and Conclusion: Our results indicate that increase in the severity of dysplasia correlates with increase in expression of p53 and p63. Increased immunoexpression of p53 and p63 may help in identifying those cases of epithelial dysplasia which can transform into carcinomas. p53 alongwith p63 is a better indicator of progression of dysplasia toward malignancy rather than p53/p63 alone. This can serve as a useful prognostic indicator and also an indicator of treatment outcome.


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